Plasmid_Backbone

Part:BBa_K1256002:Design

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-NB-Cm-MN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found at 1076
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3145
    Illegal NheI site found at 221
    Illegal SpeI site found at 1077
    Illegal PstI site found at 1091
    Illegal NotI site found at 1084
    Illegal NotI site found at 2228
    Illegal NotI site found at 3151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3145
    Illegal BamHI site found at 933
    Illegal XhoI site found at 2108
    Illegal XhoI site found at 3021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3145
    Illegal suffix found at 1077
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3145
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3160
    Illegal SpeI site found at 1077
    Illegal PstI site found at 1091
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Source

References